Application Guide

Pre-configured Solution Packages with a Commercial Scale GOX Enzyme Modified Screen Printed Sensor for Glucose Detection in Batch and Continuous Processes

The GOX Enzyme Modified Bio-Sensor

EmStat4R Portable and Rugged Potentiostat/Galvanostat/EIS

MultiEmstat4 Potentiostat/Galvanostat/EIS

The Bio-Sensor Connector Options for PalmSens Potentiostats

Remote Controlled Linear Pump for Low Volume Solutions

Dual Channel Peristaltic Pump for Conductivity Analysis

The GOX Enzyme Modified Bio-Sensor

Amperometric Glucose Oxidase three-electrode sensor with patented structure made by thick film technology.

Description: SP-1211-GO: Glucose Oxidase (GOD) from Aspergillus Niger is immobilized on the active surface of a working electrode of amperometric substrate SP-1212 (i.e. Platinum working electrode, silver reference electrode). The diameter of the immobilized bioactive membrane is 2 mm and the mean applied activity is 1 unit/mm². This screen printed amperometric sensor has three electrodes and is formed on a corundum base. It is 2.54 cm long, 0.73 cm wide, 0.63 mm thick, and weights 0.5 grams. The diameter of the platinum working electrode area is 1.00 mm. There is a silver electrical contact at the end of each sensor and this conductive path is covered by a dielectric protection layer. A biochemically active enzymatic membrane containing cca 1 IU of Glucose Oxidase enzyme is immobilized on the surface of the working electrode surface to create the biosensor. They can be ordered in a pack of 20 sensors. The PALM-SPEHOLDER-2MM is an appropriate adapter for these sensors. View the Glucose Oxidase Biosensor technical specifications.

Application Areas: Amperometric measurement of β-D-Glucose concentration in liquid samples (from 0 °C to 45 °C).

EmStat4R Portable and Rugged Potentiostat/Galvanostat/EIS:

The EmStat4R delivers desktop performance in a rugged enclosure. The EmStat4R is a portable Battery or USB-powered Potentiostat, Galvanostat, and optional a Frequency Response Analyzer (FRA) for Electrochemical Impedance Spectroscopy (EIS). The EmStat4R is great for applications that require low currents, from 30 mA down to picoamps, such as bio-sensor applications. The EmStat4R is controlled with PSTrace for Windows, the Android app PStouch, or you can write your own MethodSCRIPT and control it from any platform or operating system. The EmStat4R is available with a cell cable or SPE connection module. Both versions can be configured with optional EIS/FRA with a maximum frequency of 200 kHz. See the EmStat4R Brochure for more information.

MultiEmstat4 Potentiostat/Galvanostat/EIS:

The MultiEmStat4 is a compact Potentiostat, Galvanostat, and optional Frequency Response Analyzer (FRA) for Electrochemical Impedance Spectroscopy (EIS) with 4, 8 or 12 channels. The MultiEmStat4 comes in two versions; the Low Range version is great for applications that require a low current range down to 1 nA, whereas the High Range version is very suitable for applications that need a maximum current of 200 mA.

The MultiEmStat4 is controlled with MultiTrace for Windows, or you can write your own MethodSCRIPT and control it from any platform or operating system. See the MultiEmStat4 Brochure for more information.

The Bio-Sensor Connector Options for PalmSens Potentiostats

PALM-SPEHOLDER-2MM: PalmSens Screen Printed Electrode Connector for Drop Based Measurements.

This economical connector is a simple device with 3 banana receptacles and a connection for the screen-printed electrodes. It is compatible with any screen-printed electrode that has 2.54 mm spacing between the contact pads.

Sensor Cable for BVT-AC1 SPE for Applications involving EC Cells

This connector fits perfectly into the cells produced by BVT and this way offers a convenient tool to fix your electrodes. The connector enables the use of the BASi provided screen printed electrodes in a classic electrochemical arrangement with a stirred vessel.

PMMA Flow Cell Measurement:

The flow cell enables the use of SP-1211-GO Screen printed sensors in a flow through arrangement. The SPE can be easily inserted into the slit of cell and tightened by closing of the door. The cell ensures the wall-jet flow around the working electrode, and it is optimized so that no air bubbles cumulate in the cell. The cell also contains the contact and output cable. With the sensors enclosed in a flow cell, it is possible to measure semi-automatically or automatically using a pump or liquid switch for sample supply. Under such conditions, maximum measurement reproducibility is ensured.

In the graph below, we can see the effect of the flow rate (from 1024 microliters/min to 4 microliters/min) of the carrier liquid (physiological solution) on the response of the glucose biosensor SP-1211-GO enclosed in a flow cell. Physiological solution (carrier and washing liquid) was fed into the flow cell by a high-pressure pump. The monitored analyte (1 mM glucose solution) was fed to the biosensor through a filling loop. It can be seen from the graph that at lower flow rates, the peak corresponding to the response of the AC1.GOD biosensor to the analyte (1 mM glucose solution) broadens.

Remote Controlled Linear Pump for Low Volume Solutions:

The linear pump consists of miniature motor with digital encoder and planetary gear, which is in aluminium body of pump. The motor drives the screw which moves the piston in syringe in both directions. The pump is designed for extremely small applications such as medical or military applications. It can be used in hand-held devices. The main advantage is no pulsation and easy connection with syringe piston. The pump can supply liquid through PMMA flow cell described above. Linear pump is delivered with optional Hamilton syringe holder. View the detailed Linear Pump Specification Sheet for more information.

Dual Channel Peristaltic Pump for Conductivity Analysis:

The pump has two channels. The flow is in one direction. The lifetime of tubing is optimized to maximal value. The pulsation is minimized for PMMA flow cell mentioned above. The integrated shaft enables fastening by standard laboratory clamps. The pump is supplied by max 12V. The rotation speed is controlled by voltage. The control unit is delivered with pump. The force on the tubing is adjusted by screw a spring. The pulsation damper can be ordered separately. View the Dual Channel Peristaltic Pump Specification Sheet for more information.