Microdialysis Training

BASi offers on-site training for a variety of surgical and experimental procedures, including microdialysis. To request a quote for training, contact us. For those who do not have the time or resources for on-site training, we offer the Laboratory Microdialysis Sampling Training Manual.

The operation and format of the Laboratory Training Manual of Microdialysis and In Vivo UF Sampling on CD-ROM is similar to a web browser, and includes it s own specialized viewer. If printed, it would contain approximately 200 pages of text and graphics. There are approximately 150 photographs and illustrations, more than 50 of which are full-color, full-screen photographs illustrating probe implantations. Contact BASi for more information. Learn microdialysis in the comfort of your own lab.

Laboratory Training Manual of Microdialysis and In Vivo UF Sampling

Chapter 1: Overview of In Vivo Bioanalytical Sampling Techniques

• 1-1. The Ideal Tool
• 1-2. Open Cannula Techniques
• 1-3. Microdialysis
• 1-4. Ultrafiltration

Chapter 2: Preparation for Microdialysis

• 2-1. Selecting a Probe
• 2-2. Instrumentation Requirements
• 2-3. Perfusion Fluids
• 2-4. Preparing and Plumbing Microdialysis Probes
• 2-5. In Vitro Recovery
• 2-6. An In Vitro Recovery Experiment

Chapter 3: Setting Up a Microdialysis System: An Overview

• 3-1. Start-Up
• 3-2. Surgery
• 3-3. Perfusion System
• 3-4. Animal Containment Systems
• 3-5. Fraction Collection
• 3-6. On-Line Injector
• 3-7. Analysis

Chapter 4: Animal Preparation and Surgery

• 4-1. Working with Living Animals: Ethical Considerations
• 4-2. Anesthesia
• 4-3. Implanting Linear and Loop Microdialysis Probes
• 4-4. Stereotaxic Surgery for Brain Microdialysis
• 4-5. Microdialysis of the Vascular System
• 4-6. Shunt Approach to Monitoring Bile

Chapter 5: The Microdialysis Experiment

• 5-1. Microdialysis of the Anesthetized Rat
• 5-2. Microdialysis of the Awake Rat
• 5-3. Using the Awake Animal Systems
• 5-4. Inserting a Brain Probe in an Awake Animal
• 5-5. Sample Handling
• 5-6. Histology

Chapter 6: Monitoring Pharmacokinetics and Drug Metabolism Using Microdialysis

• 6-1. Applications for Microdialysis in Pharmacokinetics
• 6-2. An In Vitro Metabolic Study
• 6-3. Quantitative Microdialysis
• 6-4. Monitoring Pharmacokinetics of Acetaminophen in Subcutaneous Tissue
• 6-5. Monitoring Acetaminophen Metabolism On-Line with a Liquid Chromatograph
• 6-6. Data Interpretation and Determination of Pharmacokinetic Parameters

Chapter 7: In Vivo Ultrafiltration

• 7-1. Introduction
• 7-2. Physiological Basis of In Vivo Ultrafiltration
• 7-3. Practical Considerations

Chapter 8: Setting Up the Ultrafiltration System

• 8-1. System Set-Up
• 8-2. UF Probe Preparation and Implantation
• 8-3. Laboratory Experiments

Chapter 9: References

• 9-1. Perfusion Fluids
• 9-2. In Vitro Recovery and Estimation Techniques
• 9-3. Anesthesia
• 9-4. Stereotaxic Surgery
• 9-5. Ultrafiltration