In vivo ultrafiltration sampling probes use loops of membrane connected to a single collection tube. They are defined by the number of loops of membrane and the length of membrane in each loop. For example, the model UF-3-12 probe has 3 loops of membrane and each loop contains 12 cm of membrane. Use a probe like this if you want to get maximum flow and study extracellular analytes in a uniform tissue such as subcutaneous tissue. Since the membrane is formed into a loop, the loop length is half the membrane length. So in a UF-3-12 probe, the length of the loop is 6 cm. Other probe models are named in the same manner: UF-1-2 has one loop containing 2 cm of membrane, UF-3-8 has three loops each containing 8 cm of membrane, etc.
Several BASi® accessories can be used for in vivo ultrafiltration sampling. These include surgical supplies, animal caging systems, FEP tubing and tubing connectors, and the Vacuum Needle Holder. The Vacuum Needle Holder is a key convenience and safety item that we strongly recommend.
In Vivo Ultrafiltration Sampling Probes Tubing: FEP, 127cm length, 650um OD Membrane: PAN. 30KDa MWCO, 320µm OD Can be used for soft tissues that are well perfused such as subcutaneous, muscle, and IP cavity. Works for small or large animals, but Reinforced UltraFiltration (RUF) probe is best for large animals. |
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MF-7023 | UF-3-12 In Vivo Ultrafiltration Sampling Probes, 3/box Volume: 29µL |
MF-7025 | UF-3-8 In Vivo Ultrafiltration Sampling Probes, 3/box Volume: 24µL |
MF-7026 | UF-3-2 In Vivo Ultrafiltration Sampling Probes, 3/box Volume: 17.5µL |
Ultrafiltration Vacuum Vial Needle Holder |
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Reinforced In Vivo Ultrafiltration Sampling Probe Tubing: Inert biocompatible plastic, 43.7cm length, 1mm OD Membrane: PAN. 30KDa MWCO, 320µm OD Can be used for soft tissues that are well perfused such as subcutaneous, muscle, and IP cavity. RUF probe is best for large animals due to reinforced connection and more robust tubing. |
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MF-7028 | RUF-3-12 Reinforced In Vivo Ultrafiltration Sampling Probes, 3/pkg. Volume: 70µL |